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Ligation reaction
DNA Ligation-1
Enter values
Total Ligation Volume [µL]
Concentration of Vector DNA [ng/µL]
Concentration of Insert DNA [ng/µL]
Length of Vector DNA [bp]
Length of Insert DNA [bp]
Total Mass of Vector DNA [ng]
Insert DNA/Vector DNA Ratio [mol/mol]
Volume of T4 DNA Ligase [µL]
Calculate
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Results in microlitre
Water:
10x Buffer:
Vector DNA
Insert DNA
Ligase:
Total Volume:
Remarks
Vector DNA:
[nmol]
Insert DNA:
[nmol]
Insert / Vector [mol/mol] ratio:
