BioCaL (Ver. 2.30.40)

I. DNA Manipulation and PCR

  1. DNA digestion 
  2. DNA Ligation-1: User specified
  3. DNA Ligation-2: Automatically calculate
  4. DNA Ligation-3: Provide 5 conditions
  5. DNA Assembly
  6. PCR reaction setup
  7. DNAP selection and PCR mutations

II. Normalisation

  1. Normalisation by mass
  2. Normalisation by molecules
  3. Normalisation by cells

III. Conversion

  1. OD260 to mass
  2. OD260 to moles
  3. Mass to  moles
  4. Moles to mass
  5. RPM to RCF
  6. RCF to RPM

IV Physical quantities

  1. DNA physical quantities
  2. RNA physical quantities

V. Melting temperature

  1. Perfect matched DNA oligos
  2. Mismatched DNA oligos
  3. DNA oligos with inosines

VI. Genetic Delivery Systems

  1. Plasmid copy number
  2. Plasmid yield
  3. Plasmid incompatibility
  4. Cosmid in vivo packaging
  5. Expected cosmid packaging efficiency
  6. Phage multiplicity of adsorption

VII. Bacterial growth

  1. Culture tracking
  2. OD alarm timer
  3. Fitness cost
  4. Conjugation rate

VIII. Lab strain references

  1. Selection antibiotics
  2. Genotypes
  3. Common markers
  4. Genes and products
  5. Essential genes
  6. Gene summary

IX. Restriction enzymes

  1. Find recognition sequences
  2. Find Type IIP enzymes
  3. Find Type IIS enzymes
  4. Codon and enzymes
  5. 2-nt palindromic ends
  6. 4-nt palindromic ends
  7. 6-nt palindromic enzyme picker