BioCaL (Ver. 2.33.48)

I. DNA Manipulation

  1. DNA digestion 
  2. DNA Ligation-1
  3. DNA Ligation-2
  4. DNA Ligation-3
  5. DNA Assembly

II. PCR

  1. PCR reaction setup
  2. PCR mutations
  3. PCR yield
  4. Replication efficiency

III. Normalisation

  1. Normalisation by mass
  2. Normalisation by molecules
  3. Normalisation by cells

IV. Conversion

  1. OD260 to mass
  2. OD260 to moles
  3. Mass to  moles
  4. Moles to mass
  5. RPM to RCF
  6. RCF to RPM

V. Physical quantities

  1. DNA physical quantities
  2. RNA physical quantities

VI. Melting temperature

  1. Perfect matched DNA oligos
  2. Mismatched DNA oligos
  3. DNA oligos with inosines

VII. Genetic Delivery Systems

  1. Plasmid copy number
  2. Plasmid yield
  3. Plasmid incompatibility
  4. Cosmid in vivo packaging
  5. Expected cosmid packaging efficiency
  6. Phage multiplicity of adsorption

VIII. Bacterial growth

  1. Culture tracking
  2. OD alarm timer
  3. Fitness cost
  4. Conjugation rate
  5. Mutation Fixation
  6. Antibiotic Survival    

IX. Lab strain references

  1. Selection antibiotics
  2. Genotypes
  3. Common markers
  4. Genes and products
  5. Essential genes
  6. Gene summary

X. Restriction enzymes

  1. Find recognition sequences
  2. Find Type IIP enzymes
  3. Find Type IIS enzymes
  4. Codon and enzymes
  5. 2-nt palindromic ends
  6. 4-nt palindromic ends
  7. 6-nt palindromic enzyme picker